Coding

Part:BBa_K909002

Designed by: Isaak Mueller   Group: iGEM12_ETH_Zurich   (2012-09-24)

Cph8 (Cph1-Envz-Fusionprotein)

Cph8 is the fusion of Cph1, a red-light sensitive phytochrome, and the EnvZ histidine kinase, which phosphorylates OmpR and thus enables its repressing function in the ompC promoter region.

Cph1 needs the chromophore Phycocyanobilin to be able to absorb red light. Phycocyanobilin can be synthesizes from heme using heme oxigenase (ho1) and ferredoxin oxireductase (PcyA). The corresponding Biobrick can be found here: BBa_K909001

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None